Dr.Pawan Prasher, P10480
Aim: To study the association between MDM2 SNP309 mutation and pterygium
Methods: In the present study, 37 pterygium patients and 49 controls were screened for MDM2 SNP309 mutation. Peripheral venous blood samples were collected from the study participants and the genomic DNA was isolated. This genomic DNA was used for polymerase chain reaction (PCR) amplification of MDM2 SNP309 mutation using the following set of primers: forward 5’-CGGGAGTTCAGGGTAAAGG-3’ and reverse 5’-TCGGAACGTGTCTGAACTTG-3’.
Results: In the pterygium group, the frequency of genotypes GG, GT and TT were 32.65%, 40.81% and 26.53%; whereas in the controls it was 27.07%, 35.13% and 37.83% respectively. There was no statistically significant difference between the two groups in the genotype frequency of MDM2 SNP309 polymorphism (p>0.05).
Conclusions: The results show no significant difference between the ptergyium patients and controls in the genotype frequency of MDM2 SNP309 polymorphism.